原文作者: , , , , .
原文摘要:Shrimp (Penaeus monodon and P. vannamei) specimens were submitted to the University of Arizona's Aquaculture Pathology Laboratory (UAZAPL) and to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL) in 2007 and 2008 from shrimp-rearing facilities in India and Indonesia for histological testing. These were found to present prominent golden to greenish-brown needle- and plate-like birefringent crystals within multifocal hemocytic granulomas in the antennal gland tubules and peritubular hemal sinuses. Their appearance was very similar to melamine-cyanuric acid-induced crystals previously described from cat and dog kidneys with melamine-associated renal failure (MARF). Significant chronic mortalities were reported from the affected P. vannamei farms in Indonesia, but were not observed in the affected P. monodon facility in India. Shrimp feed was suspected as the source of melamine due to the similarity of the shrimp antennal gland lesions to those present in MARF. 'Normal' and 'suspect' feed samples from the facilities in Indonesia and India were sent to regional laboratories for analysis. Melamine was detected in 2 of 4 feed samples from an affected Indonesian farm. Melamine was not detected in 'normal' feed from the Indian facility, but it was found in 2 'suspect' samples (Feeds A and B) at levels of 183.39 and 112.50 ppm, respectively. A bioassay of Feed A with P. vannamei at UAZAPL confirmed that the melamine-contaminated feed induced prominent granulomas in the antennal gland with the characteristic crystals within 10 d of the first feeding, experimentally confirming the direct relationship of melamine-adulterated feed to the unique pathology observed.
印度和印尼的對蝦(南美白和斑節(jié))標本2007年和2008年分別送到亞歷山大和德克薩斯,在觸角腺和血竇周圍發(fā)現(xiàn)了針狀棕綠色晶體物。這種物體非常類似三聚氰胺晶體,因為之前有報道美國的貓和狗的腎臟衰竭魚這種物質(zhì)有關(guān)。印尼的報告上指出對蝦的慢性死亡癥狀,卻找不出病因。(這個像不像我們說的偷死?),在他們的飼料中發(fā)現(xiàn)了“疑似”物質(zhì),含量分別為183.39和112.50ppm,最后并予以確認。實驗室中感染三聚氰胺的對蝦表現(xiàn)出蝦觸角腺腫大 的癥狀。亞歷山大實驗室最后確定是飼料摻進三聚氰胺導(dǎo)致的問題。
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